Microbiology lab report on Escherichia coli and Staphylococcus aureus.

Microbiology Lab ReportPractica #1BTC307LAmber AmelingmeierThursday, September 18, 2008OBJECTIVESIn this research laboratory experiment 2 arctic types of bacteria, Escherichia coli and Staphylococcus aureus, were grown on an individual basis and mixed on quadruplet divers(prenominal) types of nutrient agar-agar in order to distinguish the vary morphologies deep wipe out the colonies. Resulting data was analyzed to volunteer understanding of the using up of differing culture media and conditions for bacterial ontogenesis. RESULTSFour different agar types were use in this experiment. The first ( food) appropriateed for proceeds of some(prenominal) E. coli and S. aureus. The second agar utilize (MKL) inhibited the return of S. aureus barely allowed the harvest-tide of E. coli. The third (ash tree) way outed exploitation of both bacterial species. The one-quarter (mannitol) was found to inhibit growth of E. coli (Table 1). Table 1. mark of growth in spite of appearance the four types of agar. G = growth, NG = no growth. E. coliS. aureusMixed cultureNutrientGGGMKLGNGG ashGGGmannitolNGGGChanges in colour within the sampled cultures were withal noted. No transplants occurred within the Nutrient agar. The MKL agar exhibited a excuse change with E. coli and the mixed culture. The agar changed from red to purple, and a translucent border was present environ the colonies that exhibited growth on the dish. The ASH agar did not yield a color change. The Mannitol agar exhibited a change with S. aureus and the mixed culture, but no change with E. coli (Table 2). Table 2.

Indication of color change within the four types of agar. E. coliS. aureusMixed cultureNutrientTransparentTransparentTransparentMKLRed to PurpleRedRed to PurpleASHRedRedRedMannitolRedRed to YellowRed to YellowCONCLUSIONS AND DISCUSSIONTwo noticeable changes occurred during this experiment. First, two of the twelve inoculated Petri dishes yielded no growth. In order to explain these occurrences, we must(prenominal) first examine the differing properties of the types of agar used. Nutrient agar is enriched to allow growth of a plushy variety... If you want to get a full essay, order it on our website: Ordercustompaper.com

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